The first lecture will take place on Monday, October 6th at 12:45 in the lecture hall of Collegium Universum, Mickiewicza 2c.

The biochemistry lectures will be on every Monday, at 12:45, in  Collegium Universum lecture hall.

The laboratory classes will start on Monday, October 6nd at 8:15 am and 2:45 pm (according to dean groups) in laboratories of the Department of Medical Biochemistry (Collegium Universum, 3^rd floor).

Please, take with you lab coat, soft footwear and Biochemistry Workbook (can be borrowed in the library or print first class from Biochemistry workbook .pdf version in Recomended handbooks on website of Department of Medical Biochemistry).

SEMINARS

Subjects indicated for self-development;

They are online on the Blackboard platform.

coordinator: dr hab. Lech Romanowicz

e-mail: lech.romanowicz@umb.edu.pl
phone: +48 (85) 748 55 78

consultations:
Justyna Bączyk      thursday 9:00 - 10:00
Marta Bruczko-Goralewska       tuesday 12:30 - 13:30
Tomasz Gogiel       friday     9:00 - 10:00
Lech Romanowicz  friday     9:00 - 10:00
Anna Tokarzewicz  friday     9:00 - 10:00

Katarzyna Miniewska  thursday 9:00 - 10:00

Credit I

Proteins

Structure of amino acids

Classification of amino acids (polarity, charge):

non-polar: Gly, Ala, Val, Leu, Ile, Phe, Trp, Met, Pro

polar without charge: Ser, Thr, Tyr, Asn, Cys, Gln

acidic chains: Asp, Glu

basic chains: His, Lys, Arg

Peptide bond

Nomenclature of peptides

Biological importance of peptides (with examples)

Protein structure: I, II (alfa-helix, beta- sheet), III (myoglobin), IV (hemoglobin),

Inborn defects in protein structure – hemoglobin, collagen

collagen triple helix;

Denaturation of proteins

Properties of proteins in solution (solubility, amphoteric properties, pI)

Isolation of proteins from biological materials: Column chromatography - size exclusion chr., ion exchange chr., affinity chr., HPLC; PAGE, isoelectric focusing, the Edman reaction

Protein functions

Enzymology

Definition of metabolism, anabolism, catabolism

Equilibrium of chemical reaction

Activation energy, free energy

Mechanism of the enzymatic reaction

Enzyme active site

Enzymatic reaction velocity and its dependence on: temperature, pH, substrate concentration, allosteric effectors (positive and negative)

Maximal velocity (Vmax) and Michaelis constant (Km)

Enzyme activity - units

Inhibitors

Proenzymes and their conversion to active forms

Regulation of enzyme activity

Multifunctional enzymes on the example of: phosphofructokinase-2,

fructose 2,6-bisphosphatase, fatty acid synthase

Multienzyme complexes on the example of:

pyruvate dehydrogenase, α-ketoglutarate dehydrogenase

Enzyme specificity

Coenzymes

Isoenzymes on the example of: lactate dehydrogenase,

creatine kinase, malate dehydrogenase, glycerol-3-phosphate dehydrogenase,

hexokinase

Classification of enzymes, international enzyme code

Application of enzymes in medicine and biotechnology:

markers of disease – aminotransferases

drugs – asparaginase

reagents – urease

gene therapy

Bioenergetics

Exergonic reactions and endergonic reactions

ATP as an energy relay

High-energy compounds

Low-energy compounds

Oxidative phosphorylation

Substrate phosphorylation

Mitochondrial respiratory chain

Respiratory complexes

Transport of protons and electrons through respiratory chain

Chemiosmotic theory

Blocking the respiratory chain

Uncoupling of oxidative phosphorylation

Reactive oxygen species

Carbohydrate metabolism

Trioses, tetroses, pentoses, hexoses

Glucose, mannose, galactose, fructose

Straight-chain form, cycle form, alpha and beta form

Aerobic and anaerobic glycolysis (with structures)

Regulation of glycolysis

Energy balance of glycolysis

Oxidative decarboxylation of pyruvate

Krebs cycle (with structures)

Credit II

Gluconeogenesis (with structures)

Pentose phosphate pathway – course, biological importance

and connection to glycolysis and gluconeogenesis

Fructose metabolism and hereditary defects: essential fructosuria, hereditary fructose intolerance

Galactose metabolism and galactosemias

Derivatives of simple sugars: glycosides, uronic acids, aminohexoses, sialic acid

Disaccharides: maltose, isomaltose, sucrose (saccharose), lactose

Polysaccharides: starch, glycogen

Biosynthesis and degradation of glycogen

Regulation of glycogen metabolism

Inborn defects in glycogen metabolism

Lipids

Fatty acids – saturated and unsaturated;

Acylglycerols – structure, decomposition;

Beta-oxidation of saturated fatty acids

Energy balance of fatty acid oxidation

Glycerol metabolism

Synthesis of fatty acids