• Warsztaty

       

      1-2. LECO – Tomas Kovalczuk – 09.00-10.00

       

       

       

       

       

       

       

       

      High Performance GC- and GCxGC-TOFMS metabolomics-based approach for the discovery

      of potential cancer biomarkers in plasma. 
      (09.00-9.30)

      Abstract - pobierz

       

      Aroma Profiling of Coffee with GC, GC×GC, and TOF MS.
      (09.30-10.00)

      Abstract - pobierz

       

      The assurance of quality and validity of generated data is nowadays strongly requested for any scientific output from a metabolomic research. Such data can be only obtained with both (i) high resolution GC and (ii) high performance MS instrumentation. The workshop will be focused on the application of comprehensive two dimensional gas chromatography hyphenated to Time of Flight mass spectrometry in non-targeted analysis in metabolomic studies. Attendees will be introduced to the principles and advantages of employed techniques (GCxGC, TOF MS) along with used approaches (peak find & spectral deconvolution algorithms, GCxGC data alignment) that will be demonstrated on two case studies focused on (i) annotation of candidate cancer biomarkers in human plasma and (ii) coffee profiling.


      Slides prezetanation - download

       

      3. Anchem/Thermo Fisher Scientic – Anas Kamleh – 10.00-11.00

       

       

      Setting a new standard for analytical rigor and confident identifications in metabolomics.

       

      During the workshops the trainees will learn about semi-targeted metabolomics workflow using a new compact HRAM Orbitrap mass spectrometer designed for exceptional usability and equipped with analytical capabilities for small molecule analysis. Trainees will learn how to discover MS instrumentation designed for exceptional usability resulting in increased productivity, recognize which analytical conditions generate high quality insightful metabolomics results, and explore innovations for small molecule analysis that lead to confident and trustworthy data. The workshop is addressed to the metabolomics researchers implementing large scale analyses and MS practitioners employing small molecule untargeted approaches for comprehensive analysis, but also to scientists new to the field of metabolomics applying biomarker discovery, global profiling, general phenotyping.

       

      Slides prezetanation - download

       

      4. BIOCRATES – Manuel Kratzke – 11.00-12.40

       

       

      Standardized metabolomics kits for quantitative and reproducible results. Kit workflow
      in the laboratory and the MetIDQ™ software.

       

      During the workshops the trainees will learn how to uncover the phenotype of an individual from just a few microliters of sample material. The main subject of the workshop is the AbsoluteIDQ® p180 kit, a simple and high-throughput tool for basic, clinical, and epidemiological research. Attendees will learn about the suitability of the kit which can be used on all stages of biomedical research – from cell cultures and rodent models to large-scale epidemiological studies. Part of the training is the familiarization with the MetIDQ™ software, a part of the kit. It guides through the registration of the plate layout, sample analysis and quantification right up to the assessment of data quality and data normalization, and finally it exports for statistical evaluations.

       

      Slides prezetanation - download

       

      5. Perlan Technologies – Łukasz Nowicki – 12.40-15.40

       

       

      Targeted and non-targeted data mining techniques for exploring high resolution spectro-chromatographic data.
      Alignment, normalization and filtering the data for efficient compound identification in metabolomics research.

       

      During the workshops, trainees will learn about untargeted and targeted techniques of spectro-chromatographic data mining using Agilent MassHunter Qualitative software. When it comes to untargeted data mining, trainees will learn about the Molecular Feature Extraction algorithm for the LC-QTOF high resolution data, and the deconvolution algorithm for the GC-MS data. Workshop participants will learn about the use of compound databases and libraries in targeted data mining workflows. With the Agilent Mass Profinder software contestants will learn about spectro-chromatographic data alignment and filtering. The main goal of the workshops is to learn about the ways of simplifying screening workflows with HRAM Q-TOF MS or GC-MS with the use of latest software tools from Agilent.

       

      6. Waters – Isabel Riba – 15.45-17.00

       

       

      Ion mobility MS/MS data processing in complex matrixes.


      During the workshops, the trainees will learn how to use Vion system, automatically calibrated devices and one software platform to acquire and process high-resolution MS data that can be used in targeted or non-targeted metabolomics, proteomics and lipidomics. During the workshops, easy to use workflows will be presented based on a true experiment of samples extracted from strawberries and raspberries. An overview on Vion IMS spectrometer will be given, including IMS Q-TOF analyzer schematics, discussing Ion Mobility Spectrometry and the Collision Cross Section parameter, presenting MSe experiment and explaining Lock Mass Ion Source principles. Software UNIFI and its main functionalities will be introduced. Trainees will learn how to create analysis methods in UNIFI software, including natural products analysis, covering such aspects of features annotation as structure elucidation or possible fragmentation.

       

      Wystąpienia sponsorów

       

      Pro-Environment Marek Michalowski

       

      HCS and In Vivo imaging applications as a necessary part of targeted therapy development

       

       

       

      Pro-Environment Magdalena Muszynska

       

      Bispecific antibodies - a new look at the molecular interactions analysis

       

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