• Updated 06.03.2018 by Zakład Klinicznej Biologii Molekularnej

    Sylabus

    SYLABUS

     

                                                     Academic year  ……2017/2018……

     

     

    Name of a  course/module

    Molecular Biology

    Name of the department where the course is held

    Department of Clinical Molecular Biology

    e-Mail of the department

    zkbm@umb.edu.pl

    Faculty of

    Medicine with Division of Dentistry and Division of Medical Education in English

    Name of a field of study

    medicine

    Level of education

    First degree studies; Uniform master’s degree studies

    Language of instructions

    English

    Form of study

     

    Full time  £                               Part time £

     

    Type of course

                 Obligatory £

               Facultative £

    Year of study/semestr

     

    I £   II £   III £ IV £  V £  VI £

     

     

    1 £   2 £   3  £  4 £  5  £  6 £   7 £   8 £  9£     10  £  11 £   12 £

     

    Introductory courses with preliminary requirements

    The implementation of learning outcomes in terms of knowledge, skills and competences of the previous years of study, particucalrly biology and biochemistry

    Number of didactic hours with specification of form of conducting classes

    15 hours in this: 5 – lectures; 10 - seminars

    Assumptions and aims of the course

    The aim of the course is to acquaint the graduate with the organization of human genes and genome, molecular mechanisms of genome stability and variability, the process of the expression of the genomic information, methods that are used in molecular analysis, and the applications of the knowledge in medical practice.

    Didactic methods

    • providing knowledge in a form of lecture with multimedial presentation
    • student’s selfstudy for seminars and preparation of a presentation
    • discussion during seminars

     

    Full name of the person conducting the course

    PhD Oksana Kowalczuk

    Full name of the person responsible for teaching

    • PhD, MD Jacek Nikliński, professor

     

     

    Symbol and number of learning outcomes according to the teaching standards and other learning outcomes

    Description of directional learning outcomes

    Verification methods for achieving intended learning outcome

     

    Knowledge

     

    BW13

    The graduate knows nucleotides’ functions in the cell, primary and secondary structure of DNA and RNA and the structure of chromatin;

     

    Summarizing methods:

    - a final test in form of multiple choice questions MCO, yes/no answer or answer completion.

    Forming methods:

    - self-study and preparation of a   presentation on a particular term

    -answer the questions

    - continuous assessment by teacher of the graduate’s activity during the discussion (in points from 0 to 3)

     

    BW14

    The graduate knows functions and basic methods of research of human genome, transcriptome and proteome; describe the process of replication, reconstruction and recombination of DNA, the process of transcription, translation and degradation of DNA, RNA and proteins; concepts of regulation of gene expression;

     

    CW4

    The graduate has got the knowledge of molecular base of mutagenesis

    CW9

    The graduate has got the knowledge of elementary diagnostics of gene and chromosome mutation developing hereditary and acquired diseases as well as  neoplasm

     

     

     

    Skills

    BU9

    The g  The graduate is skilled  at using fundamental laboratory techniques, such as electrophoresis nucleic acids

    BU11

    The graduate is skilled at using database, including that of internet and getting necessary information by accessible tools

     

     

     

    Social competence

    K1

    The graduate has got the social competence to be aware of his/her own limitations and need to improve their skills continually

     

     

     

     

    ECTS points

                  1

    Student’s workload

    Form of activity

    Number of hours to complete the activity

    Classes that require the participation of the teacher

    1. Realization of the course: lectures (according to the curriculum)

    5

    1. Realization of the course: seminars (according to the curriculum)

    10

    1. Realization of the course: tutorials (according to the curriculum)

     

    1. Credit

     

    1. Participation in consultations

     

     

    Total hours:                 15

    Student’s self-study

    1. Preparation for the seminars

    5

    1. Preparation of the presentation on a particular topic

    5

    1. Preparation for a final test-credit

    5

     

    Total hours:                 15

         

     

    Course content

    Learning outcomes (symbol and numer)

    Topics

     

    BW13, BW14, CW4, CW9

     

     

     

     

     

     

     

     

     

     

     

     

    BW13, BW14, CW4, CW9, BU9, BU11, K1

     

     

     

     

     

     

     

     

     

     

     

    Lectures

    An introduction in molecular biology:

    - human genome organization,

    - molecular mechanisms of genetic stability and variability: DNA replication DNA damage and repair,

    - genetic variability: molecular mechanisms of mutation emergence, genetic recombination,

    - molecular mechanisms of gene expression,

    - regulation of gene expression,

    - analysis of gene structure and functions – an overview of molecular biology methods,

    - application of molecular biology knowledge and methods in medicine.

    Seminars

    Genetic information and the organization of the human genome. Nucleic acids as a hereditary material: DNA structure and characteristics, RNA structure and kinds. Chromatin and chromosomes. Bacterial genomes. Genomics.

    DNA replication: molecular mechanisms and proteins and enzymes that participate in the process. Telomeres and telomerase: a chromosomal ends replication problem. Replication inhibitors as  anticancer and antiviral drugs.

    The fidelity of the DNA replication. DNA damage and repair. Basic DNA repair mechanisms. Cellular DNA damage response  and its connection with cell cycle and apoptosis. Use of DNA damage factors as anticancer and antiviral drugs.

    Molecular mechanisms of genetic variability: mutation emergence, genetic recombination). Kinds of mutations. Inherited and somatic mutations. Phenotypic effects of the mutations. Genetic polymorphisms and their impact for complex diseases.

    Gene expression: molecular mechanisms and participants. An organization of  human/eukaryotic genes and bacterial genes (gene promoters, coding part, exons and introns, regulatory sequences). An impact of general transcription factors in eukaryotic gene expression.

    Regulation of gene expression – a role of transcription factors,  chromatin regulation factors, DNA methylation and noncoding regulatory RNAs.

    Basic molecular biology methods and their application in medical diagnosis and personalized therapy approach. Analysis of gene structure and functions by PCR and hybridization methods. DNA sequencing methods. Modern high throughput methods of analyzing of the genomes, transcriptomes and proteomes.

     

    Obligatory texbooks

    TA Brown. Genomes, 3e. BIOS Scientific Publisher 2006

    LA Allison. Fundamental Molecular Biology, 2e. JohnWilley&Sons, Inc 2011

    Optional textbook:

    JD Watson et al. Biology of the gene. Pearson 2013

    WS Klug et al. Concepts of Genetics. Pearson 2012

     

     

    Criteria for assessing the achieved learning outcomes and the form and conditions for receiving credit:

     

    Preparation of a multimedial presentation on a particular topic of the course. Correct answers to at least 60% of the seminar test questions and activity during the discussion. Final written test in the form of  answer completion for those students who did not get a credit for the particular seminars and correct answer for at least 60% of the answers. In the excused absence - the obligation to make up classes after consultation with the lecturer.

     

     

    Oksana Kowalczuk, PhD

    ……………………………………………………..

    (date and signature of the person preparing the syllabus)

     

     

    Jacek Nikliński, Professor, PhD, MD                                           Oksana Kowalczuk, PhD

    ………………………………………………                    ………………………………………………

           (date and signature of the Head of the                    and                                   (course coordinator)

           Department where the course is held)