Project: The response of keratinocytes membrane and intercellular signaling to cannabidiol, used as a protection against the influence of physical and chemical environmental factors
Department: Department of Inorganic and Analytical Chemistry
My supervisor: Prof. Dr. Elżbieta Skrzydlewska
Co-investigator: Dr Agnieszka Gęgotek
General description of my project
Skin keratinocytes are the main components of the epidermis. These cells are constantly exposed to external physical and chemical factors that may cause oxidative stress and inflammation. We are looking for new cytoprotective compounds, especially from the group of compounds of plant origin. An example of such compound is cannabidiol (CBD) that is an antioxidant, anti-inflammatory compound from Cannabis sativa. It is important to check whether cannabidiol can prevent the metabolic effects of keratinocytes exposed to physical and chemical factors. Therefore, the aim of this research project is to analyze CBD effect, especially changes in cell membrane conditions, in keratinocyte cell culture which is exposed to physical and chemical environmental factors .
In M.Sc project, Potential glycosylation profile was studied for human telomerase catalitic subunit (hTERT) in hepatocellular carcinoma and neuroblastoma cell lines experimentally. Additionally, potentional glycosylation and phosphorylation sites were demostrated in telomerase catalitic subunit amino acid sequences that are from other species with bioinformatic methods and, all data was compared.
Now, Proteomics, metabolomics and lipidomics approaches are interesting area with the concept of oxidative stress.