Department: Department Of Regenerative Medicine & Immune Regulation
Public PhD Defence date: November 8th, 2022
Doctoral Thesis title: Immunomodulatory Properties Of Novel Abdominoplasty Skin-Derived Acellular Dermal Matrices
Supervisors: Prof dr hab. n. med. Marcin Moniuszko (Head of Department of Regenerative Medicine and Immune Regulation, Head of Clinical Department of Allergic and Internal Diseases)
dr hab. Andrzej Eljaszewicz (Department of Regenerative Medicine and Immune Regulation)
Reviewers: prof. dr. med. Jochen Sven Utikal (Clinical Cooperation Unit on Dermato-Oncology,German Cancer Research Center)
prof. dr hab. n. med. Marta Pokrywczyńska (Department of Regenerative Medicine Nicolaus Copernicus University in Toruń)
prof. dr hab. n. med. Jacek Tabarkiewicz (Department of Human Immunology University of Rzeszów)
Current affiliation: none
Plans for the Future: Job hunting for an academic or governmental post
Abstract of the Doctoral Thesis:
Human acellular dermal matrices (hADMs) are a frequently-utilized therapy for a variety of wounds. Tissue source, processing, and sterilization may affect therapeutic potential. Here the aim of the study was to analyze the effect of 3 different skin processing methods on hADM 1. immunogenicity; 2. immune-modulatory properties ; and 3. collagen structure with co-incubated peripheral blood mononuclear cells (PBMCs).
hADM 1 induced low T cell proliferation without significant changes in the cytokine profile. In contrast, hADM 2 & 3 were characterized by higher immunogenicity and pro-inflammatory cytokine expression. Although T cell phenotype/function was not affected, changes were observed in monocytes. Finally, apical collagen IV degradation & fragment co-localization with PBMCs was observed.
In conclusion, abdominoplasty skin is suitable for hADM production, with anionic detergents inducing low-immunogenicity and beneficial immunomodulatory properties. Although further research is needed, high therapeutic potential is indicated by these results.